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. Author manuscript; available in PMC: 2013 May 11.
Published in final edited form as: Cell. 2012 May 11;149(4):832–846. doi: 10.1016/j.cell.2012.03.032

Figure 3. Ultrastructural organization of DFz2C/LamC foci (also see SF3.

Figure 3

Transmission electron micrographs of larval muscle or S2 cell nuclei containing DFz2C/LamC foci.

A- Low magnification of a focus (box; enlarged in inset) within a muscle nucleus.

B–D- High magnification of INM invaginations containing electron dense granules (g) from
  • B- larval muscle.
  • C, D- S2 cells. Dense granules appear to be bounded by membrane (arrow in D).
E–H- Immunoelectron micrographs of muscle nuclei labeled with
  • E, G- anti-LamC and 18nm gold-conjugated second antibody shown at (E) low and (G) high magnification;
  • F, H- anti-DFz2C and anti-LamC with 12nm and 18nm gold conjugated second antibody, respectively, shown at (F) low and (G) high magnification.

N= nucleus; C= cytoplasm; nu= nucleolus; h=heterochromatin; inm= inner nuclear membrane; onm= outer nuclear membrane; m= myofibrils; z= perforated z band. Calibration=0.5µm A; 0.3µm B, D; 0.4µm E, F; 0.1µm C, G, H.