Fig. 1.

Genotyping of Fads1 knockout mice. (A) Mice were cloned from an embryonic stem cell line (IST11525H2) carrying the Fads1 allele disrupted by the insertion of a gene trap vector (Omnibank Vector 76) in the first intron. The insertion disrupts transcription of the gene. (B) DNA isolated from mouse tail was genotyped by PCR. Fads1 gene product (310 bp) was found only in Wt and Het mice; V76 (Omnibank Gene Trap Vector 76) gene product (270 bp) was found only in Het and Null mice. Refer to Materials and Methods for primer sets and details describing the gene trapping technique.