Fig. 1.

Generation of (R)-2-OH FA by FA2H. GC-NCI-MS analysis of the absolute configuration of 2-OH PA in racemic 2-OH PA (A), wool wax containing enantiopure (R)-2-OH PA (B), purified (R)-2-OH PA (C), and (S)-2-OH PA (D), CHO cells overexpressing hFA2H (E), and control CHO cells with vector (F). The enzyme expression in CHO cells was evaluated by immunoblotting (IB) using antibody recognizing hFA2H (G). In conclusion, FA2H is stereospecific for the production of the (R)-enantiomer of 2-hydroxy FAs.