Fig. 3.

Stereospecific rescuing effect of 2-OH PA on accelerated raft fluidity induced by FA2H depletion. 3T3-L1 adipocytes were treated with a negative control siRNA (CTL) or an siRNA recognizing FA2H (FA2H). Twenty-four hours post transfection, 50 μM (R)-2-OH PA (2R) or (S)-2-OH PA (2S) was added as indicated. Labeling with Alex 488-CTxB and FRAP measurements were performed. A: Selected images from a confocal FRAP experiment in adipocytes labeled with Alexa 488-CTxB at room temperature. B: Kinetics of recovery for Alexa 488-CTxB. Each curve represents means ± SE from 10 experiments. The fluorescence recovery of individual experiments is shown in supplementary Fig. I. C: Calculated Mfs. D: Diffusion coefficients (D). **^a P < 0.01 (compared with CTL). **^b P < 0.01 (compared with FA2H). NS, nonsignificant (compared with FA2H).