Fig. 6.

Regulation of Cer and HexCer containing 2-OH PA by FA2H and 2-OH PA enantiomers. 3T3-L1 adipocytes were treated with a negative control siRNA (CTL) or an siRNA recognizing FA2H (FA2H). Twenty-four hours post transfection, 50 μM (R)-2-OH PA (2R) or (S)-2-OH PA (2S) was added as indicated. C16:0 2-OH Cer and C16:0 2-OH HexCer were quantified by LC ESI-MS/MS, and results are the means ± SE of three independent experiments. *^a P < 0.05 (compared with CTL); *^b P < 0.05 (compared with FA2H); **^b P < 0.01 (compared with FA2H).