Fig. 4.

Ezetimibe-mediated changes in jejunal expression of key cholesterol homeostatic genes. Analysis of pooled data from four independent studies is presented. Ages 11- to 14-week-old male C57BL/6J mice were fed chow diets with or without 0.003% ezetimibe in the diet for three or four days. Total RNA from jejunocytes (four or five mice per group in each of four studies) was separately isolated; equal amounts of RNA from each mouse was pooled and subjected to quantitative real-time PCR using cyclophilin as the invariant control. Each value represents the relative mRNA level or protein level in ezetimibe-treated mice compared with control mice who did not receive ezetimibe, which was normalized to 1.0. Each bar represents the mean ± SEM from the pooled data from four studies; overall n = 18 mice. The levels of statistical significance between the control and ezetimibe-treated groups are indicated (paired Student t-test; *P < 0.05, **P < 0.01).