TABLE 2.
Measurement of sterols, oxysterols, and secosteroids
| Average (RSE) | Median | Range | Pooled Plasma Average (RSE)† | NIST SRM Plasma Average | Spike Recovery (%)† | |
| Oxysterol (ng/ml)* | ||||||
| 7αHC (x) | 158.7 (3.2) | 153.6 | 33–435 | 56.86 (10.6) | 91.8 | 104.3 |
| 7α,27HC (c) | 10 (2.4) | 9.6 | 2–22.8 | 9.24 (5.3) | 9.6 | 99.6 |
| 25OH VD3 (i) | 24.3 (2.8) | 25.6 | 0–57.6 | 9.32 (1.4) | 20.7 | 97.1 |
| 25HC (l) | 11.8 (2.4) | 11.0 | 2.6–28.2 | 6.1 (3.9) | 5.7 | 106.3 |
| 24HC (m) | 56.1 (2.1) | 53.6 | 24–117 | 46.13 (3.3) | 44.6 | 101.4 |
| 27HC (o) | 151.4 (2.0) | 144.1 | 60–390 | 124.6 (2.0) | 131.0 | 100.9 |
| 4βHC (k) | 53.1 (2.4) | 48.9 | 16.7–144 | 43.9 (3.0) | 31.4 | 104.0 |
| 24oxoC (n) | 6.0 (2.2) | 5.6 | 2.6–12.1 | 5.1 (13.5) | 3.7 | 109.0 |
| 24/25EC (r) | 1.8 (4.4) | 1.5 | 0.2–9.1 | 1.5 (6.7) | 1.8 | 100.1 |
| 7oxoC (a) | 84 (4.5) | 66.5 | 25.7–360.8 | 40.54 (18.3) | 24.0 | 104.6 |
| Sterol (ng/ml)* | ||||||
| Chol (M)§ | 184.0 | 182.5 | 112–319 | 120.7 | 145.0 | N/A |
| Zymo (D) | 24.8 (6.5) | 16.9 | 0–139 | 19.7 (1.6) | 32.2 | 96.9 |
| Desm (E) | 713.4 (3.3) | 692.0 | 37–1,800 | 623 (0.8) | 722.2 | 97.1 |
| Lano (O) | 203.2 (6.4) | 151.2 | 35–1,480 | 101.2 (1.1) | 123.9 | 94.6 |
| 24-DiHL (U) | 20.8 (13) | 10.2 | 2.4–347 | 5.5 (1.6) | 8.5 | 96.0 |
| 7-DHC (H) | 803.2 (2.8) | 744.7 | 424–2,488 | 510 (1.1) | 1,137.8 | 85.0 |
| Cholestanol (P) | 2,913.9 (2.3) | 2827.6 | 160–6,200 | 2,526 (0.6) | 2,581.1 | 95.9 |
| 14-DML (N) | 517 (3.6) | 456.0 | 14.5–1,915 | 305 (1.1) | 451.1 | 107.3 |
| Lath | 3,892 (7.1) | 3,388 | 166–14,460 | 3,078 (3.9) | 2,080 | 106.5 |
| Camp (Q) | 3,831.4 (3.8) | 3,546.1 | 808–16,400 | 2,502 (0.7) | 2,645.6 | 111.0 |
Results from a cohort of 200 subjects from the Cooper Institute in Dallas, TX. Data are nanograms per milliliter except for cholesterol, which is milligrams per deciliter. NIST SRM, pooled plasma, and spike recovery are included for comparison as well as for QA/QC analysis. Letters in parentheses correspond to chromatographic peak labels in Fig. 1. Error values in parentheses represent the relative standard error (RSE).
*N = 200.
†N = 6.
§Milligrams per deciliter measured by clinical chemistry assay.