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Knockdown of CCN1 in OE21 ESCC cells (OE2CCN1−) reduces the level of β-catenin. (a) Under a dual filter, the shRNA transfected cells display bright red fluorescence due to expression of RFP, while β-catenin is identified in green by FITC-conjugated antibody. (b) In the same view under FITC filter, the shRNA transfected cells display low level of β-catenin compared to nontransfected cells. (c) Western blot analysis shows reduced expression of β-catenin in OE21CCN1− cells.
