Figure 5.

Integrin α ₁₁ mediates CCN1-induced β-catenin activation and translocation. (a) Het-1A cells were pretreated with either preimmune serum (control) or anti-integrin α ₁₁ antibody cultured in the absence (−) or presence (+) of CCN1 overnight. β-catenin was pulled down from the cells lysates through immunoprecipitation, subsequently analyzed by Western blot for expression of total β-catenin, active β-catenin (dephosphorylated at Ser37 or Thr41), and Ser/Thr-phosphorylated β-catenin. (b) Immunofluorescence microscopic analysis shows that blocking integrin α ₁₁ with a specific antibody prevents CCN1 to induce β-catenin translocation.