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rde-11 mutants fail to accumulate secondary siRNAs in response to exogenous pos-1 RNAi. (A and B) Ratio of pos-1 siRNA reads in rde-11 mutants to WT (WT = 1.0) from total non-treated (A) or TAP treated (B) RNA. (C) pos-1 siRNA size and 5′ nucleotide distribution in WT and rde-11 mutant worms. (D) siRNA distribution across the pos-1 genomic region in WT and rde-11 mutants. The shaded region indicates the sequence corresponding to the pos-1 dsRNA used for RNAi.
