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. 2012 May 2;32(18):6072–6080. doi: 10.1523/JNEUROSCI.6486-11.2012

Figure 4.

Figure 4.

Loss of TH in VTA does not prevent the D1-mediated increase in glutamate transmission with AMPH stimulation. A, Left, Confocal images from an AAV-THsiRNA and an AAV-SCR-injected mouse acquired at 5× represent mean fluorescence from each group. TH staining is observed in the VTA of AAV-SCR-injected mice but is virtually absent in mice injected with AAV-THsiRNA. Scale bar, 0.5 mm. Right, Coronal drawing depicts bilateral location of VTA and surgical targeting adapted from Reference Mouse Brain Atlas: Allen Institute for Brain Sciences. B, Plot shows AMPH increases glutamate transmission in slices from both AAV-THsiRNA and AAV-SCR-injected mice (THsiRNA, 119 ± 4%; n = 4; SCR, 120 ± 8%; n = 6; p > 0.05). C, Plot shows SKF increases glutamate transmission in slices from AAV-THsiRNA-injected mice (AAV-THsiRNA SKF, 135 ± 1%; p > 0.05; n = 4). AMPH is replotted for comparison. Error bars indicate SEM.