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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Gastroenterology. 2011 Nov 19;142(3):543–551.e7. doi: 10.1053/j.gastro.2011.11.020

Figure 4. 4-HNE activates stathmin.

Figure 4

A, Laser scanning confocal microscopy shows normal microtubules (red) in untreated HCT116 cells (i) and degraded microtubules in cells treated with supernatants from E. faecalis-infected macrophages (ii–iv) or purified 4-HNE (v and vi); blue represents DNA stain. Treated cells contain lagging chromosomes (ii), anaphase bridging (iii and vi), multipolar spindle (iv), and spindle failure at metaphase (v). B, Western blots show changes in two isoforms (19 and 20 kDa) of p-stathmin (Ser16) in HCT116 cells treated with 4-HNE and nocodazole. C, By normalizing to β-actin, the 19 kDa isoform significantly decreases 0.5–6 hrs following 4-HNE treatment (red) compared to untreated control (blue). This decrease occurs 24 hrs after nocodazole treatment (green). D, The 20 kDa isoform increases in cells treated with 4-HNE or nocodazole.