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. Author manuscript; available in PMC: 2012 Jun 11.
Published in final edited form as: J Biol Inorg Chem. 2010 Mar 11;15(5):737–748. doi: 10.1007/s00775-010-0642-1

Fig. 3.

Fig. 3

Characterization of KP1019 and KP1339 apoptosis. a Loss of mitochondrial membrane potential after 24-h treatment was determined by 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining. Increase of the green fluorescent apoptotic populations of KB-3-1 cells at the drug concentrations indicated (cells in the lower right field) are indicated (30,000 events were analyzed in total per group). b Effects of 30-min pretreatment with the radical scavenger N-acetylcysteine (NAC) on the anticancer activity of KP1019, KP1339, and H2O2 (positive control) were analyzed after 72-h drug exposure by an EZ4U kit