Figure 3.
PDGFRβ promotes GSC growth. Representative FACS plots of 08-387 (A) and 4121 (B) cells demonstrating isolation of PDGFRβhigh cells. Growth of PDGFRβhigh and PDGFRβlow cells isolated via FACS from 08-387 (C) or 4121 (D) cells over time was measured using adenosine triphosphate (ATP) content in accordance with the cell titer assay. PDGFRβhigh cells grow faster than PDGFRβlow cells. Growth of 08-387 (E) or 08-322 (F) GSCs expressing two different shRNAs directed against PDGFRβ (shPDGFRβ I and shPDGFRβ II) was lower than GSCs expressing nontargeting shRNA (shNT) as measured over time using the cell titer assay. (G) Growth of GSCs exposed to increasing concentrations of PDGFRβ inhibitor III was decreased in the cell titer assay. (H) Representative images of GSCs exposed to increasing concentrations of PDGFRβ inhibitor III.