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. 2012 May 31;8(5):761–777. doi: 10.7150/ijbs.4036

Figure 1.

Figure 1

Characterization of specific antibodies against TTP family proteins. (A) Alignment of the amino acid sequences of the three mouse TTP family members. The TZF domain and the C terminus are highly conserved. Black dots denote CCCH residues in the TZF domain. Underlining indicates regions used to produce antiserum for each protein. The amino acids which are the same in three family proteins are highlighted in black, and those only the same in two members are highlighted in gray. (B) Coomassie blue staining of purified TTP family members expressed in E. coli with a maltose-binding protein tag at the N terminus and a 6×His tag at the C terminus for antiserum production. L1: ZFP36L1; L2: ZFP36L2. (C) HEK293T cells expressed GFP-TTP, GFP-ZFP36L1 (L1), and GFP-ZFP36L2 (L2). WCEs (40 µg) were applied to each lane for immunoblotting with anti-TTP, anti-ZFP36L1, and anti-ZFP36L2. Antiserum dilution was 1:1000.