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. Author manuscript; available in PMC: 2012 Jun 11.
Published in final edited form as: J Biol Chem. 1995 Dec 22;270(51):30441–30447. doi: 10.1074/jbc.270.51.30441

Fig. 3. Alkaline phosphatase sensitivity of cleavage products.

Fig. 3

Complexes C199 (lanes 3 and 4), G200 (lanes 5 and 6), and Ia (lanes 7 and 8) were radiolabeled at position C199 and treated with rat liver SII (phosphocellulose fraction, 0.5 μ g) and 7 mM MgCl2 for 10 min at 28 °C. Cleavage products were separated from the complexes by centrifugation, butanol-extracted, dried, dissolved in 9 μl of 1 mM MgCl2, and treated with (lanes 4, 6, and 8) or without (lanes 3, 5, and 7) 0.05 unit of calf intestinal alkaline phosphatase (Boehringer Mannheim) for 10 min at 37 °C. Samples were dried and resolved on a 25% polyacrylamide gel. Lanes 1, 2, and M are chemically synthesized, 32P-labeled pApC, pCpG, and pCpGpUpUpUpUpU, respectively.