Figure 2.

Number of protein spots differentially accumulated during Arabidopsis seed germination. For each time interval, a total number of 475 spots are considered. The normalized volume for each spot was determined in three biological repetitions. The log2 ratio was calculated between 0 and 24 h as well as between 24 and 48 h. When the spot was not detected in one of the time point, a negligible spot abundance value was arbitrary assigned to calculate the log2ratio. (A) Number of protein spots whose abundance is decreasing (log2 ratio < −1) between germination sensu stricto (0–24 h), radicle protrusion (24–48 h) or at both time intervals of seed imbibition. (B) Number of protein spots whose abundance is increasing (log2 ratio >1) between germination sensu stricto (0–24 h), radicle protrusion (24–48 h) or at both time intervals of seed imbibition.