Table 3.

Specific GAPDH activity in lotus and tomato roots upon lumichrome treatment assayed under non-phosphorylating and phosphorylating conditions.

	Fraction	L. japonicus		S. lycopersicum
		Control	Root application	Control	Root application
Non-phosphorylating GAPDH		19.72 ± 1.20	14.24 ± 0.71	4.26 ± 0.29	3.67 ± 0.14
Phosphorylating NADP-GAPDH	Crude	1.39 ± 0.33	0.59 ± 0.18	0.85 ± 0.07	1.43 ± 0.35
	Plastid	0.16 ± 0.07	0.42 ± 0.17	0.21 ± 0.03	0.86 ± 0.25
	Supernatant	1.52 ± 0.08	1.08 ± 0.23	0.96 ± 0.24	1.89 ± 0.42
Phosphorylating NAD-GAPDH	Crude	9.62 ± 1.79	10.35 ± 1.23	24.87 ± 1.69	42.39 ± 4.03
	Plastid	0.97 ± 0.01	2.23 ± 0.73	1.56 ± 0.39	4.32 ± 0.57
	Supernatant	11.63 ± 1.08	9.25 ± 1.67	15.83 ± 1.97	29.44 ± 8.09

Under phosphorylating conditions, enzyme activity of crude extracts, plastid fractions and resultant supernatant fractions were assayed from root material. The values are the mean ± SE (n = 6) and values in bold type were determined by Student’s t-test to be significantly different (P < 0.05) from the respective untreated control. Non-phosphorylating and phosphorylating NADP-dependent enzyme activity is presented in nmol NAD(H/P) min⁻¹ mg⁻¹ protein, while phosphorylating NAD-dependent GAPDH values are expressed in μmol NAD min⁻¹ mg⁻¹ protein.