Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jun 11;3:102. doi: 10.3389/fgene.2012.00102

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2012 Naito and Ui-Tei.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

PMC Copyright notice

Schematic representation for selecting functional and off-target effect-reduced siRNAs. Selection of highly functional siRNA by Ui-Tei, Reynolds, Amarzguioui rules, or the combination of them (STEP 1). Selection of siRNAs with low stability in the seed-target duplexes (STEP 2). Elimination of siRNAs with near-perfect matched sequences to non-target genes (STEP 3). In each rule, the nucleotide position indicates the number of nucleotide counted from 5′ terminal of the guide strand. : A/U at position 1. G/C at position 19. 4 to 7 A/Us in positions 1-7. No GC stretch ≥ 10. : GC content (30%-52%). A/U ≥ 3 at positions 1-5. Absence of internal repeats. A at position 1. A at position 17. U at position 10. Not G/C at position 1. Not G at position 7. : An asymmetry in the stability of the duplex ends (measured as the A/U differential of the three terminal basepairs at either end of the duplex). G or C at position 19. A or U at position 1. A or U at position 14. Not U at position 19. Not G at position 1.

Inline graphic — Schematic representation for selecting functional and off-target effect-reduced siRNAs. Selection of highly functional siRNA by Ui-Tei, Reynolds, Amarzguioui rules, or the combination of them (STEP 1). Selection of siRNAs with low stability in the seed-target duplexes (STEP 2). Elimination of siRNAs with near-perfect matched sequences to non-target genes (STEP 3). In each rule, the nucleotide position indicates the number of nucleotide counted from 5′ terminal of the guide strand. : A/U at position 1. G/C at position 19. 4 to 7 A/Us in positions 1-7. No GC stretch ≥ 10. : GC content (30%-52%). A/U ≥ 3 at positions 1-5. Absence of internal repeats. A at position 1. A at position 17. U at position 10. Not G/C at position 1. Not G at position 7. : An asymmetry in the stability of the duplex ends (measured as the A/U differential of the three terminal basepairs at either end of the duplex). G or C at position 19. A or U at position 1. A or U at position 14. Not U at position 19. Not G at position 1.