Figure 6.

The minimal bactericidal concentration was determined by growing E. coli or S. aureus in the presence of 2a (Fig 5a) or 3a (Fig 5b) at 10 ug/ml over 24 hours. At each time point dilutions of the cell cultures were plated onto Mueller Hinton plates and the number of colony forming units were used to calculate the cell concentration. The results show that nearly all bacteria were killed by the 2a or 3a compounds by 18 hours. Control cells incubated in the absence of compounds 2a or 3a continued to grow as expected.