Figure 2.
Functional agonism by the tested in an assay of adenylate cyclase in membranes of CHO cells expressing the hA3AR. Activity of the 2-(3,4-difluorophenylethynyl)-N6-3-chlorobenzyl-5′-N-methyluronamide-(N)-methanocarba analogue 31 (EC50 value 1.2±0.7 nM). The full agonist 5′-N-ethyluronamidoadenosine 48 was included for comparison (representing 100% efficacy). The experiment was repeated three times, and the average is shown.