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. 2012 Jun 4;209(6):1105–1119. doi: 10.1084/jem.20112258

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© 2012 Franzke et al.

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Figure 5. — Infiltrating skin macrophages do not cause the skin barrier defects in A17^ΔKC mice. For depletion of dermal macrophages, A17^ΔKC mice were subcutaneously injected with clodronate-loaded liposomes (A17^ΔKC + Clod) or PBS-loaded liposomes (A17^ΔKC + PBS, Ctrl) into the back skin. (A) Flow cytometry of skin macrophages at P19 gated for CD11b⁺Ly6G⁻ and further analyzed for skin macrophages (CD45⁺F4/80⁺; n = 5). (B) Immunofluorescence staining of A17^ΔKC skin with anti-F4/80 antibodies to detect macrophages (top) and for TGM activity (bottom, arrows in Ctrl; n ≥ 5 per group). Bars, 20 µm. (C) TEWL from the back skin of A17^ΔKC mice injected with clodronate-loaded (A17^ΔKC + Clod) or control (A17^ΔKC + PBS) liposomes and from control mice was detected from P8 to P19 (n ≥ 5 per group). Data are mean ± SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001.