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. 2012 Jun 4;209(6):1135–1152. doi: 10.1084/jem.20120030

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© 2012 Satpathy et al.

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Figure 3. — Zbtb46^gfp specifically identifies cDCs in the mouse. Cells from Zbtb46^+/+, Zbtb46^gfp/+, and Zbtb46^gfp/gfp were harvested from the spleen, SLNs, MLNs, and thymus as indicated and stained for CD11c, MHCII, CD172, CD24, and CD45. (A) Shown are two-color histograms for the indicated markers after gating for CD45 expression. Numbers represent the percentage of cells in the indicated gate. (B) Shown are two-color histograms for the indicated markers derived from a GFP⁺ gate as indicated in A from Zbtb46^gfp/gfp mice. (C) Shown are single-color histograms for GFP expression of cells from Zbtb46^+/+ mice (WT) or cells from Zbtb46^gfp/+ mice stained to identify cDCs (MHCII^hiCD11c^hi), resident cDCs (MHCII^intCD11c^hi), migratory cDCs (MHCII^hiCD11c^int/lo), pDCs (CD317⁺B220⁺), RPMs (F4/80⁺ autofluorescent), monocytes (CD11b^hiCD11c⁻MHCII⁻Ly6G⁻), granulocytes (Ly6G⁺CD11b⁺), CD8⁺ T cells (CD8α⁺CD11c⁻), CD4⁺ T cells (CD4⁺CD11c⁻), B cells (B220⁺MHCII⁺), NK cells (NKp46⁺), DP thymocytes (CD4⁺CD8⁺Lin⁻), or DN thymocytes (CD4⁻CD8⁻Lin⁻). (D) BM cells from Zbtb46^+/+ or Zbtb46^gfp/+ mice were cultured with 100 ng/ml Flt3L for 6–9 d as indicated, harvested, and stained for CD11c, MHCII, and CD317. Gates identifying pDCs, cDCs, and precursors are indicated in two-color histograms for CD317 and MHCII expression on the left. Shown on the right are two-color histograms for CD11c and GFP expression for pDCs, cDCs, and precursor cells. All data in this figure are representative of three to five independent experiments using at least two mice per group.