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. 2012 Jun 4;209(6):1135–1152. doi: 10.1084/jem.20120030

Figure 4.

Figure 4.

GM-CSF–derived cDCs express Zbtb46gfp. (A) BM cells from C57BL/6 mice were treated with 20 ng/ml GM-CSF or M-CSF, and cultures were harvested on day 7 and divided into adherent and nonadherent fractions. Nonadherent CD11c+ cDCs from the GM-CSF–treated cultures were purified by positive selection, and microarray analysis was performed on cDCs (GM-CSF CD11c+) and both adherent populations (Adh) for the indicated cultures. Shown is the expression value of the Zbtb46 probe set. (B) BM monocytes were purified by negative selection using antibodies for Ly6G, B220, CD4, CD8α, DX5, CD11c, Ter119, and CD117, resulting in a fraction of cells that were >90% pure for expression of CD11b and Ly6C. Cells were cultured with 20 ng/ml GM-CSF for the indicated number of hours and harvested, and microarray analysis was performed. Shown is the expression value of the Zbtb46 probe set. Data in A and B are from one experimental replicate obtained from cells pooled from three mice. (C) Monocytes were purified from BM by cell sorting as CD11b+Ly6C+Ly6G−CD11c−MHCII−Siglec-H− cells and cultured in 20 ng/ml GM-CSF with or without 20 ng/ml IL-4 as indicated and analyzed after 4 d for expression of Zbtb46gfp and MHCII. Data are representative of one experiment in which each sample was performed in triplicate. Numbers represent the percentage of cells in the indicated gate.