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. 2012 Jun 4;209(6):1167–1181. doi: 10.1084/jem.20120340

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© 2012 Hoeffel et al.

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Figure 8. — Fetal liver monocytes differentiate into LC precursors. Monocytes were purified from E13.5–E14.5 fetal liver of Cx3cr1^gfp/+CD45.1⁺ mice and adoptively transferred in utero into unconditioned E13.5–E14.5 CD45.2⁺ congenic embryos. (A–C) Flow cytometry analysis of the progeny of adoptively transferred CD45.1⁺ monocytes (A) in the blood (B) and skin (C) 3 h after injection. (A) Gating strategy (top) for monocytes among fetal liver leukocytes (DAPI⁻CD45⁺), purity after sorting (middle), and profile of expression for Gr-1 and CX3CR1/GFP (bottom). (B and C) Percentage CD45.1⁺ donor-derived monocytes among total CD45⁺ cells (top), expression of CD11b and F4/80 (middle), and Gr-1 and CX3CR1/GFP (bottom) among indicated populations. (D) Percentage cells derived from donor monocytes for each injected embryo (n = 7) in E14.5 blood and skin 3 h after transfer. Control represents noninjected embryos (n = 9). Bars show means of data from one representative experiment of three. (E) Percentage populations P1 (CD11b^hiF480^lo) and P2 (CD11b^loF480^hi) on gated DAPI⁻CD11b⁺F480⁺CD45.1⁺ donor-derived cells isolated from the skin at indicated time points after transfer (E14.5) and their corresponding profile of expression for Gr-1 and CX3CR1/GFP. (F) Percentage population P2 on gated DAPI⁻CD11b⁺F480⁺CD45.1⁺ donor derived cells isolated from the skin at indicated time points after transfer. Error bars represent mean ± SEM of pooled data from two experiments (n = 3 to 8). (G) Graph shows the percentage of skin CD11b⁺F4/80⁺ cells derived from donor fetal liver monocytes (white squares) or donor fetal liver Ter119⁺ erythroid progenitors (gray squares; n = 4–11) for each injected embryo at the indicated time points. Bars represent means of data at each time point. ***, P < 0.0001; *, P < 0.01.