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. Author manuscript; available in PMC: 2012 Jun 11.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Oct 7;30(12):2597–2603. doi: 10.1161/ATVBAHA.110.215681

Figure 3.

Figure 3

Notch 1 IC or HRT-1 overex-pression blocks inhibition of SMC proliferation by EtOH. A and B, SMCs were transfected without (mock) or with constitutively active Notch 1 IC (A) or hHRT-1 (B) and treated with EtOH for 24 hours; PCNA protein levels were measured by Western blot as an index of proliferation. Blots were reprobed for β-actin or GAPDH as a loading control. A, *P<0.05 vs mock control (0-mmol/L EtOH). A representative Western blot is shown, with cumulative data (n=3). B, Top, A representative Western blot of 3 is shown; bottom, growth curves for control and EtOH-treated cells, with or without HRT-1 overexpression.