Table 1: PCR Conditions used for amplifying the TNF-α regions.

TNF -238/-244		TNF -308		TNF -857		TNF -863
1 cycle	94° for 3 min	1 cycle	94° for 4 min	1 cycle	94° for 4 min	1 cycle	94° for 4 min
30 cycle	94° for 40 sec	30 cycle	94° for 60 sec	30 cycle	94° for 40 sec	30 cycle	94° for 45 sec
	53° for 40 sec		56° for 60 sec		56.5° for 40 sec		53° for 45 sec
	72° for 55 sec		72° for 60 sec		72° for 55 sec		72° for 60 sec
1 cycle	72° for 6 min	1 cycle	72° for 8 min	1 cycle	72° for 6 min	1 cycle	72° for 6 min