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| SNP | PCR product Size | Enzyme | Digest pattern | |
| TNF-238 | (G →A) | 239 bp | Bgl II | A (216, 23 bp ) |
| G (239 bp ) | ||||
| TNF-244 | (G →A) | 239 bp | Bsaj I | G (169,78 bp ) |
| A (239 ) | ||||
| TNF-308 | (G →A) | 107 bp | Nco I | G (87, 20 bp ) |
| A (107 bp ) | ||||
| TNF-857 | (C →T) | 127 bp | Tai I | C (109, 18 bp ) |
| T (127 bp ) | ||||
| TNF-863 | (C →A) | 126 bp | Tai I | A (105, 21 bp ) |
| C (126 bp ) | ||||
