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. 2012 Jun 3;2012:976972. doi: 10.1155/2012/976972

Figure 3.

Figure 3

(a, b) Multiplex PCR amplification of MSCRAMMs and biofilm genes of MRSA and MSSA clinical isolates. (a) is related to the amplified bands of 10 genes of icaA, icaD, icaB, icaC, bbp, fib, eno, clfA, clfB, ebbps, and finbA at 1100, 900, 574, 404, 301, 288, 203, 180, and 128 bp, respectively, in one tube reaction, all these genes were determined in 5 different clones of MRSA and MSSA: MRSA-spa-t091(L1), MSSA-spa-t159(L2), MSSA-spa-t14413(L3), MSSA-spa t3204(L4), MSSA-spa-t4085(L5). (b) is related to all the 10 amplified genes mentioned in (a) except bbp, which was replaced with finbB at 523 bp and was found in 16 different clones of MRSA: MRSA-spa-t932(L1), t037(L2, L3, L4), t421(L5, L6, L7), t41509(L8, L9, L10), t2575(L11), t138(L12, L13), and t4213(L14, L15, L16). 16sRNA at product size 455 bp was used as internal positive control. M is DNA ladder marker.