Table 1.

PCR target genes and primers used in this work.

Genes	Nucleotide sequence of primers	PCR annealing temperature (°C)	Amplicon size (bp)	Reference
icaA (intercellular adhesion gene)	5-ACACTTGCTGGCGCAGTCAA-3	55°C	188	Rohde et al., 2001 [6]
	5-TCTGGAACCAACATCCAACA-3
icaD (intercellular adhesion gene)	5-ATGGTCAAGCCCAGACAGAG-3	55°C	198	Rohde et al., 2001 [6]
	5-AGTATTTTCAATGTTTAAAGCAA-3
icaB (intercellular adhesion gene)	5-AGAATCGTGAAGTATAGAAAATT-3	55°C*	900	Kiem et al., 2004 [7]
	5-TCTAATCTTTTTCATGGAATCCGT-3′
icaC (intercellular adhesion gene)	5-ATGGGACGGATTCCATGAAAAAGA-3	55°C*	1100	Kiem et al., 2004 [7]
	5-TAATAAGCATTAATGTTCAATT-3
eno (laminin binding protein)	5-ACGTGCAGCAGCTGACT-3	55°C	301	Tristan et al., 2003 [8]
	5-CAACAGCATCTTCAGTACCTTC-3
ebpS (elastin binding protein)	5-CATCCAGAACCAATCGAAGAC-3	55°C	180	Tristan et al., 2003 [8]
	5- AGTTACATCATCATGTTTATCTTTTG-3
fnbA (fibronectin binding protein A)	5-CATAAATTGGGAGCAGCATCA-3	55°C*	128	Vancraeynest et al., 2004 [9]
	5- ATCAGCAGCTGAATTCCCATT-3
fnbB (fibronectin binding protein B)	5-GTAACAGCTAATGGTCGAATTGATACT-3	55°C	523	Tristan et al., 2003 [8]
	5-CAAGTTCGATAGGAGTACTATGTTC-3
fib (fibrinogen binding protein)	5-CTACAACTACAATTGCGTCAACAG-3	55°C	405	Tristan et al., 2003 [8]
	5-GCTCTTGTAAGACCATTTTCTTCAC-3
clfA (clumping factor A)	5-ATTGGCGTGGCTTCAGTGCT-3	55°C	288	Tristan et al., 2003 [8]
	5-CGTTTCTTCCGTAGTTGCATTTG-3
clfB (clumping factor B)	5-ACATCAGTAATAGTAGGGGCAAC-3	55°C	203	Tristan et al., 2003 [8]
	5-TTCGCACTGTTTGTGTTTGCAC-3
bbp (bone sialoprotein binding protein)	5-AACTACATCTAGTACTCAACAACAG-3	55°C	574	Tristan et al., 2003 [8]
	5-ATGTGCTTGAATAACACCATCATCT-3
cna (collagen binding protein)	5-AAAGCGTTGCCTAGTGGAGA-3	55°C	192	Montanaro et al., 1999 [10]
	5-AGTGCCTTCCCAAACCTTTT-3

*Optimized PCR annealing temperature program in this study at 55°C.