Table 4.
Comparison of gene expression levels among 6 different clones of spa types by measuring the band intensities of RT-PCR amplicons using nanodrop spectrophotometer (ng/μL).
| gene |
aMLST ST-121 spa-t3204 |
aMLST ST-121 spa-t14413 |
aMLST ST-121 spa-t159 |
bMLST ST-239 spa-t4150 |
bMLST ST-239 spa-t421 |
bMLST ST-239 spa-t037 |
|---|---|---|---|---|---|---|
| icaA | 598.11 ± 1.06 | 698.19 ± 3.21 | 815.04 ± 3.1 | 796.19 ± 1.01 | 716.05 ± 0.74 | 515.06 ± 0.59 |
| icaD | 577.87 ± 12.14 | 675.76 ± 2.01 | 991.55 ± 1.1 | 875.88 ± 0.8 | 892.41 ± 2.33 | 691.37 ± 0.78 |
| icaB | 708.88 ± 2.04 | 803.77 ± 2.31 | 764.13 ± 0.8 | 601.87 ± 0.45 | 865.52 ± 1.02 | 864.48 ± 2.86 |
| icaC | 591.95 ± 1.8 | 699.88 ± 3.02 | 600.64 ± 12.1 | 998.94 ± 0.66 | 9001.65 ± 1.52 | 567.59 ± 3.22 |
| fnbA | 684.56 ± 5.7 | 785.49 ± 1.33 | 904.95 ± 14.1 | 488.55 ± 2.41 | 805.86 ± 1.10 | 504.71 ± 1.81 |
| fnbB c | 749.72 ± 2.11 | 909.17 ± 4.2 | 800.84 ± 1.23 | |||
| Fib | 544.35 ± 21.01 | 643.72 ± 9.02 | 783.13 ± 1.9 | 949.37 ± 3.01 | 681.78 ± 3.11 | 483.42 ± 12.1 |
| cna | 560.85 ± 14.1 | 661.93 ± 1.60 | 660.35 ± 0.6 | 360.84 ± 0.8 | 559.89 ± 2.81 | 360.81 ± 9.22 |
| eno | 461.06 ± 5.1 | 565.15 ± 11.5 | 558.28 ± 0.4 | 464.05 ± 0.77 | 454.92 ± 0.62 | 458.94 ± 0.96 |
| ebps | 542.18 ± 1.3 | 641.28 ± 2.12 | 506.78 ± 0.88 | 543.17 ± 9.1 | 405.14 ± 0.73 | 806.37 ± 0.89 |
| bbp d | 580.52 ± 2.4 | 684.39 ± 2.11 | 474.13 ± 2.03 | |||
| clfA | 955.26 ± 11.8 | 1051.49 ± 1.01 | 466.45 ± 2.01 | 552.29 ± 0.33 | 765.54 ± 2.39 | 766.68 ± 0.99 |
| clfB | 759.44 ± 2.1 | 859.67 ± 2.10 | 459.50 ± 1.04 | 755.41 ± 2.62 | 556.67 ± 0.59 | 659.81 ± 1.78 |
aThree clones of MLST sequence type ST121 belonging to clonal cluster 121 (CC121) with different spa types were showed to be weakly adherent to the surface of polystyrene microtiter plat after 48 h growth, these clones were found positive for 12 genes except the fnbB genec. bThree clones of MLST sequence type ST239 belonging to clonal cluster 8 (CC8) with different spa types were showed to be strongly adherent to the surface of polystyrene microtiter plat after 48 h growth, these clones were found positive for 12 genes except the bbp gened. Every six different clones showed statistically significant difference (P ≤ 0.05) in the intensity of RT-PCR amplified products when fewer cycles were used (25) in the linear phase of amplification for most transcripts. The replicates of Rt-PCR products were done in duplicate.