Table 2.
Cycle threshold values for the 10-fold dilution row of DNA from 1 million Blastocystis organisms/200 μl elution buffer
| Probe |
CT values for duplicates at dilution ofa: |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| 1 × 100 | 1 × 10−1 | 1 × 10−2 | 1 × 10−3 | 1 × 10−4 | 1 × 10−5 | 1 × 10−6 | 1 × 10−7 | 1 × 10−8 | |
| Blasto | 21.90/21.89 | 25.15/25.22 | 28.67/28.67 | 31.04/30.97 | 34.84/35.11 | 36.82/40.91 | 39.21/38.12 | 40.93/UD | UD/UD |
| IPC | UD/UD | UD/UD | UD/47.26 | 36.56/38.64 | 35.17/35.11 | 35.18/35.70 | 36.76/36.03 | 36.70/34.95 | 35.98/37.15 |
a
DNAs were tested in duplicates to test for reproducibility. UD, undetermined (i.e., signal absent).