Proposed flowchart for identification of rifabutin (RFB) susceptibility. In this study, 104 rifabutin-susceptible isolates were identified among 800 multidrug-resistant Mycobacterium tuberculosis isolates. Shown is a suggested algorithm for identification of rifabutin susceptibility. *, group 1 represents samples missing only wt2, only wt3 and wt4, or only wt5 and wt6; **, group 2 represents samples simultaneously missing wt7, mut2A, and mut2B.