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. 2012 Jun;50(6):2126–2128. doi: 10.1128/JCM.06829-11

Table 1.

Sequences of the primers and probes used in this study

Primer or probe Sequencea Locationb
Primers
    AgD-F1 5′CGGGCCGGCTGTTCTTCT3′ 1158–1175
    AgD-F2 5′CGGGCCGGCTACTCTTCT3′ 1158–1175
    AgD-R 5′AAGGAAGGCCCTCGAGAACA3′ 1287–1268
Probes
    TaqMan-MGB 5′CTCTTCTTCCTCCYTGCTGA3′ 1215–1234
    LNA-BHQc 5′CTTCTTCCTCCYTGCTGA3′ 1217–1234
    dLy86 5′CTCTTCCTCCTCCGCGCTGA3′ 1215–1234
    dLy20 5′CTCTTCTTCTTCCTTGCTCA3′ 1215–1234
    dLy131 5′CTCTTCCTCTTCCTTGCTGA3′ 1215–1234
a

TaqMan-MGB and LNA-BHQ probe sequences were aligned with patients' sequences correctly detected by the LNA-BHQ probe but not by the TaqMan-MGB probe. Boldface letters in the two forward primer sequences indicate differences. Boldface letters in the probe sequences show the differences between the probe and patient sequences.

b

Numbering refers to the HDV-1 genome (accession number M21012).

c

LNA nucleotides are underlined.