Table 1.
Comparison of molecular techniques with conventional methods for diagnosis of pulmonary tuberculosisa
| Mycobacterium species | Conventional method |
Molecular technique |
MAS-PCR analysis |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Biochemical test |
Genotype |
Multiplex PCR |
PCR-RFLP analysis |
Organism | No.b | Resistance pattern | Mutation site | |||||
| Organism | No.b | Resistance pattern | No.b | Organism | No.b | Organism | No.b | |||||
| M. tuberculosis | M. tuberculosis | 25 | IR + RR + ER | 1 | M. tuberculosis | 99 | M. tuberculosis | 102 | M. tuberculosis | 1 | IR + RR + ER | 315, −15, 306, 516 |
| M IR | 2 | M. tuberculosis | 4 | M IR | 315 | |||||||
| ER +RR | 1 | M. tuberculosis | 4 | M IR | −15 | |||||||
| M. tuberculosis | 2 | M RR | 526 | |||||||||
| M. tuberculosis | 1 | M RR | 531 | |||||||||
| M. tuberculosis | 1 | M RR | 516, 531 | |||||||||
| M. tuberculosis | 2 | M RR | 516 | |||||||||
| M. tuberculosis | 2 | M ER | 306 | |||||||||
| M. tuberculosis | 1 | IR + RR | 315, −15, 531 | |||||||||
| M. tuberculosis | 1 | IR + RR | −15, 516 | |||||||||
| M. tuberculosis | 1 | IR + RR | −15, 526 | |||||||||
| M. tuberculosis | 1 | IR + RR | 315, 516, 526 | |||||||||
| M. tuberculosis | 1 | IR + RR | 315, 531 | |||||||||
| M. tuberculosis | 1 | IR + RR | −15, 531 | |||||||||
| M. tuberculosis | 2 | ER + IR | −15, 306 | |||||||||
| M. tuberculosis | 1 | ER +RR | 306, 526 | |||||||||
| M. tuberculosis | 1 | ER +RR | 306, 516, 531 | |||||||||
| M. bovis | M. bovis | 1 | – | – | M. bovis | 7 | M. bovis subsp. bovis | 8 | M. bovis subsp. bovis | 1 | M IR | −15 |
| M. bovis subsp. bovis | 1 | M IR | 315, −15 | |||||||||
| M. bovis subsp. bovis | 1 | RR | 516, 531 | |||||||||
| M. avium | M. avium subsp. avium | 5 | M. avium subsp. avium | 5 | M. avium subsp. avium | 1 | M IR | −15 | ||||
| M. avium subsp. intracellulare | 3 | M. avium subsp. intracellulare | 1 | M IR | −15 | |||||||
| M. kansasi | M. kansasi | 1 | IR + RR | 1 | NTM | 20 | M. kansasii | 9 | M. kansasi | 1 | M IR | −15 |
| M. kansasi | 1 | M IR | 315 | |||||||||
| M. kansasi | 1 | RR | 526 | |||||||||
| M. kansasi | 1 | IR + RR | −15, 516, 531 | |||||||||
| M. fortuitum | M. fortuitum | 1 | – | – | M. fortuitum | 2 | M. fortuitum | 1 | M IR | 315 | ||
| M. fortuitum | 1 | M IR | −15 | |||||||||
| M. chelonae | M. chelonae subsp. abscessus | 3 | M. chelonae subsp. abscessus | 1 | IR +RR +ER | 315, 306, 531 | ||||||
| M. chelonae subsp. abscessus | 1 | IR + RR | 315, −15, 526 | |||||||||
| M. chelonae subsp. abscessus | 1 | ER +IR | 315, −15, 306 | |||||||||
| M. malmoense | M. malmoense | 1 | M. malmoense | 1 | M ER | 306 | ||||||
| M. szulgai | M. szulgai | 1 | M. szulgai | 1 | M ER | 306 | ||||||
| M. terrae | M. terrae | 1 | – | – | – | |||||||
a
IR + RR + ER, resistance to isoniazid, rifampin, and ethambutol; M IR, monoresistance to isoniazid; IR + RR, resistance to both isoniazid and rifampin; IR + ER, resistance to both isoniazid and ethambutol; M RR, monoresistance to rifampin; RR + ER, resistance to both rifampin and ethambutol; M ER, monoresistance to ethambutol; NTM, nontuberculous mycobacteria.
b
No., number of isolates showing positive results.