Table 1.
EHV-1 ORF30 specific primer/probe sets used in E1 and E2 allelic-discrimination rPCR assays
| Assay | Primer or probe (sense) | Probe specificitya | Location (nt)b | Sequence (5′–3′) |
|---|---|---|---|---|
| E1 | E1Fwd (positive) | NA | 2229–2245 | TCT GGC CGG GCT TCA AC |
| E1Rev (negative) | NA | 2276–2294 | TTT GGT CAC CCA CCT CGA A | |
| E1PrA2254 (positive) | A2254 | 2247–2262 | 5HEX-ATC CGT CAA CTA CTC G-BHQ2a | |
| E1PrG2254 (positive) | G2254 | 2247–2262 | 6∼FAM-ATC CGT CGA CTA CTC G-BHQ1 | |
| E2 | E2Fwd (positive) | NA | 2204–2218 | CCA CCC TGG CGC TCG |
| E2Rev (negative) | NA | 2328–2348 | AGC CAG TCG CGC AGC AAG ATG | |
| E2PrA2254 (positive) | A2254 | 2246–2261 | VIC-CAT CCG TCA ACT ACT C-MGB | |
| E2PrG2254 (positive) | G2254 | 2248–2261 | 6-FAM-CAT CCG TCG ACT ACT C-MGB |
a
NA, not applicable.
b
Nucleotides are numbered according to GenBank accession number AY464052.