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. 2012 Jun;50(6):2111–2113. doi: 10.1128/JCM.00220-12

Fig 1.

Fig 1

Babesia ovata-specific AMA-1 PCR assay development. A new PCR method was developed for the diagnostic detection of B. ovata, based on the B. ovata AMA-1 gene. (A) PCR specificity. Lanes 1 to 13 represent genomic DNA samples from B. ovata, Babesia bigemina, Babesia bovis, Theileria annulata, T. orientalis, Trypanosoma brucei gambiense, Trypanosoma evansi, T. theileri, Anaplasma marginale, Anaplasma bovis, Anaplasma centrale, Anaplasma phagocytophilum, and normal bovine blood, respectively. (B) PCR sensitivity. 10-fold serial dilutions of B. ovata genomic DNA extracted from in vitro culture. Lanes 1 to 8 represent 10 ng/μl, 1 ng/μl, 100 pg/μl, 10 pg/μl, 1 pg/μl, 100 fg/μl, 10 fg/μl, and 1 fg/μl, respectively. An uninfected bovine DNA sample was included as a negative control (lane 9). M, 100-bp DNA marker ladder (both panels).