Fig 1.
Fluorescence emission versus cycle number from results of the MycAssay Pneumocystis real-time PCR assay demonstrating examples of samples with an exponential amplification pattern with CT values of <39.0 interpreted as positive (a), nonexponential amplification with CT values of <39.0 interpreted as negative (b), and a nonexponential amplification pattern with undetermined CT values interpreted as negative (c). An arrowed line indicates the threshold of 10,000 specified by the MycAssay Pneumocystis kit. Rn is the ratio of the emission intensity of the reporter dye divided by the emission intensity of ROX at each cycle. Delta Rn is the Rn value baseline subtracted on a per-well basis.