Table 1.

Comparison of detection of P. jirovecii in respiratory samples by the MycAssay Pneumocystis real-time PCR assay and a laboratory standard of IFA, cdc2 real-time PCR, or mtLSU conventional PCR and sequencing

MycAssay mtLSU real-time PCR result	No. of specimensb
	IFA			cdc2 real-time PCR			mtLSU conventional PCR			Laboratory gold standard
	Pos.	Neg.	Total	Pos.	Neg.	Total	Pos.	Neg.	Total	Pos.	Neg.	Total
Positive	9	18	27	16	11	27	26	1	27	27	0	27
Negative	0	68a	68	0	68a	68	0	68a	68	0	68a	68
Total	9	86	95	16	79	95	26	69	95	27	68	95

^aTwo samples had a MycAssay Pneumocystis real-time PCR assay C_T of <39.0 but did not have an exponential amplification pattern.

^bPos., positive; Neg., negative.