Fig 4.
The RNase H activity of Tf1 RT in the absence or presence of increasing Tf1 IN/RT molar ratios. The soluble RNase H assay that measures the release of [3H]AMP from [3H]poly(rA)-poly(dT) substrate into the TCA-soluble fraction was carried out as described in Materials and Methods. The activity of Tf1 RT by itself with no IN present has a value of 1, and all other IN/RT ratios exhibit activities relative to this value (relative activity [RA]). The numbers represent the results of three independent experiments after subtracting the nonspecific backgrounds (with the indicated SD).