Fig 6.

The removal of the nonhomologous MLV primer by Tf1 RT. All reactions were performed with a constant amount of wild-type Tf1 RT in the absence (lane 2) or the presence of Tf1 IN, with increasing molar ratios of IN to RT ranging from 0.25:1 to 2:1 (lanes 3 to 6). 2(HI), a 2:1 IN/RT ratio of Tf1 RT with heat-inactivated Tf1 IN (lane 7); 2(NR), with the same concentration of Tf1 IN with no RT present (lane 8); MLV RT, a 2:1 Tf1 IN/MLV RT molar ratio (lane 9); HIV-1 RT, a 2:1 Tf1 IN/HIV-1 RT ratio (lane 10). S, substrate only (lane 1).