Table 3.
Viral loads in hind limb tissue from 6- to 7-day-old CD1 mice after SC infection with WT or mutant CHIKV strain LR or vaccine strain 181/25
| Virus straina | Day 2 postinfection |
Day 4 postinfection |
Day 6 postinfection |
Day 8 postinfection |
||||
|---|---|---|---|---|---|---|---|---|
| No. of animals positive/total no. inoculated | Mean titerb of positive animals ± SD | No. of animals positive/total no. inoculated | Mean titerb of positive animals ± SD | No. of animals positive/total no. inoculated | Mean titerb of positive animals ± SD | No. of animals positive/total no. inoculated | Mean titerb of positive animals ± SD | |
| LR | 2/3 | 3.3 ± 2.2 | 2/3 | 4.3 ± 3.0 | 2/3 | 2.3 ± 1.2 | 1/3 | 2.1 ± 2.0 |
| LR/nsP1 | 2/2 | 3.1 ± 0.1 | 1/2 | 2.6 ± 2.3 | 0/2 | ≤ 0.9 | 0/2 | ≤ 0.9 |
| LR/6K | 3/3 | 5.0 ± 1.4 | 3/3 | 6.8 ± 0.2 | 3/3 | 6.9 ± 0.9 | 1/3 | 2.3 ± 2.5 |
| LR/E1 | 3/3 | 4.3 ± 1.3 | 3/3 | 5.8 ± 1.1 | 1/3 | 1.5 ± 1.1 | 0/3 | ≤ 0.9 |
| LR/E2-12 | 3/3 | 4.3 ± 1.0 | 2/3 | 3.5 ± 2.4 | 1/3 | 1.9 ± 1.8 | 0/3 | ≤ 0.9 |
| LR/E2-82 | 0/2 | ≤ 0.9 | 0/2 | ≤ 0.9 | 0/2 | ≤ 0.9 | 0/2 | ≤ 0.9 |
| 181/25 | 1/2 | 3.7 ± 4.0 | 2/2 | 5.2 ± 1.2 | 2/2 | 4.2 ± 0.4 | 1/2 | 3.1 ± 3.0 |
a
See Fig. 1b for strain designations.
b
Titers are in log10 PFU/g of tissue, and the limit of detection of the assay was 0.9 log10 PFU//g of tissue.