Table 2.
Summary of the phenotypes of the ICP0 RING finger mutants analyzed in this study
| Phenotypea | wt | FXEb | RF | ICP0 RING finger mutant with the following mutation: |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A117T | V118A | T120P | D121V | I140N | W146A | M147R | Q148E | N151D | P154T | L155P | L160R | ||||
| Y2H | + | − | − | + | − | − | + | − | + | − | + | + | − | − | − |
| UBE2D1 | |||||||||||||||
| Auto-ub | + | − | − | + | − | − | + | − | + | +/− | + | +/− | − | − | − |
| Poly-ub | + | − | − | + | + | +/− | + | + | + | +/− | + | + | +/− | − | + |
| UBE2E1 | |||||||||||||||
| Auto-ub | + | − | − | + | − | − | +/− | − | + | − | + | +/− | − | − | − |
| Poly-ub | + | − | − | + | +/− | − | + | + | + | − | + | + | − | − | − |
| FK2 | + | − | − | + | +/− | − | + | +/− | + | +/− | + | +/− | +/− | − | − |
| PML degradation | + | −* | − | + | + | − | + | +/− | + | +/− | + | +/− | − | − | − |
| Complementation | + | −* | − | + | + | − | + | − | + | − | + | +/− | − | − | − |
| Derepression | + | −* | − | + | − | − | + | − | + | − | +/− | − | − | − | − |
a
The mutants' respective abilities to interact with E2 ubiquitin-conjugating enzymes (as determined by Y2H analysis [Fig. 2B]), catalyze the formation of polyubiquitin chains (poly-ub) and autoubiquitinate (auto-ub) in the presence of UBE2D1 or UBE2E1 stimulate the colocalization of conjugated ubiquitin (FK2), degrade PML, complement the PFE of dl1403/CMVlacZ, and derepress lacZ gene expression from quiescent in1374 viral genomes are indicated as follows: +, wt-like; −, ICP0-RF-like; +/−, intermediate.
b
Asterisks indicate previously characterized phenotypes as described in reference 33.