Fig 10.

Activation of cytokines and TLR following intravenous Ad3-GFP. (A and B) Cytokine array. Small intestine and colon samples were combined and lysed. Lysates were analyzed for a set of 40 mouse cytokines and chemokines using the Proteome profiler array kit from R&D Systems. n = 3. (A) Representative image of dot blot from tissue of one mouse per group. The array includes positive controls (“pos.”) provided by the manufacturer. Cyto-/chemokines that significantly differ in their concentrations (P < 0.05) in both groups are highlighted in the upper dot blot. (B) Quantification of signals from all animals (n = 3). The blots were scanned and signal intensity was measured. MIG, CXCL9, MIP-1, CCL3. (Notably, as antibodies specific for each individual cytokine are used in this cytokine array, absolute levels for different cytokines cannot be compared. This implies that the levels of IL-1ra, a protein that inhibits IL-1α and -β activity, might be lower than those of IL-1α and IL-1β.) (C) Immunofluorescence analysis on intestine sections of hDSG2-positive mice for TLR6 and E-cadherin (left panel) and TLR6 and F4/80 (right panel). Scale bar, 20 μm. Representative sections are shown.