Fig 4.

One-bead-one-compound (OBOC) approach for the identification of glycopeptide epitopes (synthetic strategy 3). Design of a 13-mer random OBOC library with a fixed GalNAc-Thr at position 6 and with randomized amino acid residues at positions X₋₂ to X₊₃ linked to a cleavable linker as shown. The library was sequentially screened with a pool of HSV-2-positive (n = 3) sera (1:100 dilution), and the sequences of the isolated hits were deconvoluted with mass spectrometry. The glycosylated amino acids are underlined and in boldface, and the hit frequency of each sequence is shown in parentheses in the table.