Fig 11.

Reduction of cell survival upon angiogenin knockdown. (A) 293T cells were transfected with control sh-GFP and sh-Renilla (RL) plasmids or two sh-ANG (sh-ANG1 and sh-ANG2) plasmids, and ANG knockdown was checked by separating the lysates with a 12.5% gel and performing Western blotting for ANG. (B) ANG knockdown was also checked by real-time RT-PCR in sh-GFP- and sh-ANG1-transduced BCBL-1 cells. (C to E) BCBL-1 cells transduced with either sh-GFP or sh-ANG1 were subjected to Western blotting for p53, p-p53, p21, Bcl-2, Bax, caspase 3, and β-actin. Fold changes are indicated. (F) BCBL cells transduced with either sh-GFP or sh-ANG1 were stained with YO-PRO dye and analyzed by FACS for the percentage of live cells. Values shown represent the means ± standard deviations from three independent experiments. ***, P < 0.005.