Fig 7.

Effects of 3 days of neomycin treatment on LANA-1, ANG, and p53 (A to C) nuclear extracts were prepared from BCBL-1 cells that were either left untreated or treated with 200 μM neomycin for 3 days. Treated and untreated nuclear lysates were subjected to IP with rabbit anti-ANG IgG antibodies, separated with a 7.5% gel, and subjected to Western blotting for LANA-1 (A); subjected to IP with rabbit anti-LANA-1 IgG antibodies, separated in a 10% gel, and subjected to Western blotting for p53 (B); or subjected to IP with rabbit anti-ANG IgG antibodies and Western blotting for p53 (C). Input controls for ANG, LANA-1, and p53 are shown for all IP reactions. (D and E) BCBL-1 cells were treated with neomycin as described above, and the expression of ORF73 and ORF50 genes was determined by real-time RT-PCR. Values shown represent the means ± standard deviations from three independent experiments. *, P < 0.05; ***, P < 0.005. BJAB (F and G) and BCBL-1 (H and I) cells were either left untreated or were treated with 200 or 500 μM neomycin for 3 days, and the expression of p53 and p21 genes was measured by real-time RT-PCR.