Fig 6.

A3G editing of HIV-1 ΔVif and HTLV-1. (A) Supernatants of cells cotransfected with HIV-1 ΔVif and GFP or A3G was used to infect TZM-bl cells. Proviral DNA was extracted, and a 1,905-bp fragment in HIV-1 pol was PCR amplified and purified from gel. PCR fragments were subsequently used as a template in the 3DPCR with a range of denaturing temperatures (80.5 to 83.2°C) and analyzed on agarose gels. The denaturing temperature (Td) (80.5, 80.9, 81.2, 81.5, 81.2, and 83.2°C) is indicated by the height of the black triangle above the gel. HTLV-1 editing was performed as described in the legend to Fig. 5. (B) PCR products amplified at the lowest denaturing temperature were cloned, and eight individual clones were sequenced. Red lines indicate GG-to-AG mutations, blue lines indicate GA-to-AA mutations, pink lines indicate GT-to-AT mutations, green lines indicate GC-to-AC mutations, and black lines indicate non-G-to-A mutations. (C) Pie chart representation of the relative dinucleotide preferences of A3G for HIV-1 and HTLV-1.