Fig 5.

Identification of a recombinant VP1/2 fragment sharing properties with the native carboxyl-terminal isoform. (A) Illustration of full-length VP1/2 (top) and two cloned fragments (VP1/2N and VP1/2C) fused to GFP. DUB, deubiquitinase; PRR, proline-rich region; ARR, alanine-rich region; CBD, capsid binding domain; G, green fluorescent protein. (B) Vero cells were transiently transfected with VP1/2N or VP1/2C expression constructs, and total cell lysates were separated by SDS-PAGE. Protein migration was compared to that of endogenous VP1/2 species from Vero cells infected with PRV-GS909 (G-VP1/2) or PRV-GS1903 (VP1/2-G). Molecular size markers (in kDa) are indicated to the left of each blot. (C) Localization of GFP emissions from a Vero cell transiently expressing VP1/2C. (D) Nuclear GFP emissions from a Vero cell transiently expressing VP1/2C and subsequently infected with the RFP-capsid virus, PRV-GS847.