Fig 5.

Ligand activation of PPARβ/δ inhibits expression of promitotic proteins in 308 cells. 308 cells were cultured for up to 72 h and treated with GW0742 to activate PPARβ/δ for various periods of time as illustrated by the arrows. (A and B) Western blot analysis of mitosis-related proteins (A) or RB, E2F1, and E2F4 (B) in 308 cells treated as described above. Expression levels were normalized to β-actin and are presented as fold change relative to control DMSO results. (C) 308 cells were cultured for 24, 48, or 72 after transfection with an E2F-luciferase reporter construct. Luciferase activity was measured and normalized to renilla as an internal control. For all data sets, n = 3 independent samples per treatment group. Values represent means ± SEM.